草菇开伞相关基因反义表达载体构建

Antisense Expression Vector Construction of Volvariella volvacea Fruitbody Opening Related Sequence

谢宝贵　 罗联忠

摘　要：以质粒pCAMBIA1301和pBI121为基础构建草菇表达载体pCB.把已克隆的草菇开伞相关基因DNA片段正反向插入pCB,获得pCB-opn15和pCB-opn39,经测序证实,pCB-opn15为正向插入,pCB-opn39为反向插入.

关键词：草菇; 表达载体; 开伞相关基因; 反义基因

分类号：S646.130.1　文献标识码：A

文章编号：1005-9873(2005)02-0009-05

基金项目：福建省自然科学基金重点项目"草菇开伞基因克隆及反义基因构建"(编号:B 0120002)的部分内容

作者简介：谢宝贵(1962-),男,2004毕业于福建农林大学作物遗传育种专业,博士,教授,主要从事食用菌的遗传育种及分子生物学研究,发表主笔论文15篇.

作者单位：谢宝贵（福建农林大学菌物研究中心,福州,350002）　

　　　　　罗联忠（福建农林大学菌物研究中心,福州,350002;中国热带农业科学院热带作物品种资源研究所,海南省儋州市,571737）　

参考文献：

[1]Tentler S, Palas J, Enderlin C, et al. Inhibition of Neurospora crassa growth by a glucan synthaser-1 antisense construct[J]. Current Microbiology, 1997,34:303～308.

[2]Diatchenko L, Lau YFC, Campbell AP, et al. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries[J]. Proc Natl Acad Sci USA, 1996,93(12):6025～6030.

[3]Bhairi SM, Staples RC. Transient expression of the β-glucuronidase gene introduced into Uromyces appendiculatus uredospores by particle bombardment[J]. Phytopathology, 1992,82(9):986～989.

[4]Chen X, Stone M, Schlagnhaufer C, et al. A fruiting body tissue method for efficient Agrobacterium mediated transformation of Agaricus bisporus[J]. Applied and Environmental Microbiology, 2000,66(10):4510～4513.

[5]Smith CJ, Watson CF,Morris PC, et al, Inheritance and effect on ripening of antisense polygalacturonase genes in transgenic tomatoes[J]. Plant Mol Biol, 1990,14(3): 369～379.

食用菌学报

ACTA EDULIS FUNGI

2005 Vol.12 No.2 P.9-13